Campylobacter jejuni Modulates Reactive Oxygen Species Production and NADPH Oxidase 1 Expression in Human Intestinal Epithelial Cells

Figure 2

C. jejuni modulates NOX1 expression in T84 and Caco-2 cells. qRT-PCR showing expression of NOX1 in (a) T84 and (b) Caco-2 cells. (c) RT-PCR showing expression of NOX1 in uninfected T84 and Caco-2 cells. GAPDH was used as an internal control. (d) RT-PCR showing expression of NOX1 in T84 cells infected with C. jejuni for 24 hours and (e) relative mRNA levels as a percentage from RT-PCR data. (f) Western blotting showing NOX1 in T84 cells infected with C. jejuni for 24 hours and (g) relative protein level as a percentage from Western blotting. Asterisks denote a statistically significant difference (; ; ).

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