Campylobacter jejuni Modulates Reactive Oxygen Species Production and NADPH Oxidase 1 Expression in Human Intestinal Epithelial Cells

Figure 6

The effect of DPI on C. jejuni interaction, invasion, and intracellular survival. T84 and Caco-2 cells were pretreated with 10 μM of DPI for 1 hour and infected with C. jejuni for 3 hours. (a) T84 and (b) Caco-2 cells were washed with PBS and lysed, and the numbers of interacting bacteria were assessed. (c, d) For invasion assay, after infection with C. jejuni, IECs were incubated with gentamicin (150 μg/ml) for 2 hours to kill extracellular bacteria and then lysed, and the numbers of intracellular bacteria were assessed. (e, f) For intracellular survival assay, 2 hours of gentamicin treatment was followed by further incubation with gentamicin (10 μg/ml) for 18 hours. Then, cells were lysed, and the numbers of intracellular bacteria were assessed. Experiments were repeated in three biological and three technical replicates. Asterisks denote a statistically significant difference (; ; ).

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