Knockout of Noxa with CRISPR/Cas9 Increases Host Resistance to Influenza Virus Infection

Figure 2

Noxa knockout inhibits influenza virus replication. (a) Microscopy observations of virus-induced cytopathic effect reduction in Noxa-knockout cells 24 h postinfection. Phase contrast microscopy was used to observe and photograph cells. Scale bar, 100 μm. (b) Flow cytometry showed the apoptotic cell death in Noxa-knockout cells 24 h postinfection. (c) Viral titer in the supernatant of the indicated cell lines was detected with a TCID50 assay 24 h postinfection. (d) Influenza viral protein expression was analyzed via western blotting in Noxa-knockout cells 24 h postinfection.

(a)

(b)

(c)

(d)