Mycobacterium avium Infection of Multinucleated Giant Cells Reveals Association of Bacterial Survival to Autophagy and Cholesterol Utilization

Figure 3

Uptake and survival of MGC-passaged M. avium A5 in THP-1 cells. (a) Percent uptake of M. avium A5 inoculums in THP-1 cells after 1 h of infection. (b) Percent reduction of uptake by THP-1 cells treated with either wortmannin for 2 h or a CD11b antibody for 1 h, prior to infection. (c) Fold change relative to 1 h.p.i (dashed line) of bacterial survival by plate or MGC-passaged M. avium in THP-1 macrophages over 6 days. “Plate” refers to M. avium A5 grown on a 7H10 plate, and “MGC-passaged” refers to M. avium A5 that was collected from infected-MGCs at 72 h.p.i. Data are representative of three independent experiments. Statistical comparisons: , , and .

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