G₁ Cell Cycle Arrest Is Induced by the Fourth Extracellular Loop of Meningococcal PorA in Epithelial and Endothelial Cells

Figure 2

Migration of epithelial cells is inhibited by PorA-Loop4 peptide. HCT116 cell monolayers were mechanically wounded, treated, and wound closure monitored over a 60 h time course. (a) Representative light microscope images showing wound closure following treatment with media only (untreated), or PorA-Loop4 (at 5μM or 50 μM final conc.) at 0, 24, and 60 h. (b) Percentage wound closure of epithelial cells treated with PorA-Loop4 (at 5 μM or 50 μM final conc.) or a scrambled Loop4 peptide (PorA-Scr Loop4; at 50 μM). Wound width was determined by measuring 5 different points across the same field of view on the same wound at each time point. All data are normalized to the time 0 wound width and expressed as mean percentage (%) wound closure (+ SEM) from three independent experiments, each carried out in triplicate. Significantly different compared to untreated (: one-way ANOVA with Dunnett’s test applied post hoc).

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