G<sub>1</sub> Cell Cycle Arrest Is Induced by the Fourth Extracellular Loop of Meningococcal PorA in Epithelial and Endothelial Cells

<div>PorA-Loop4 causes an increase in the proportion of cells in G<sub>1</sub> phase. HBMECs were treated with either PorA-Loop4 (50 <i>μ</i>M), PorA-Scr Loop4 (50 <i>μ</i>M), and nocodazole (50 <i>μ</i>g ml<sup>-1</sup>) or were serum deprived for 24 h. During the final 2 h of incubation, 20 <i>μ</i>M of BrdU was added to all cells. Cells were harvested, fixed, and costained with anti-BrdU (Alexa 488) and PI and analyzed for cell cycle by flow cytometry. Proportions of cell cycle phases (a) G<sub>1</sub>, (b) S-phase, and (c) G<sub>2</sub>/M are expressed as percentage of total population for treated cells. Each bar represents at least three independent experiments, and each experiment measured a minimum 10,000 events. 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443 166 443 312C443 466 390 635 241 635ZM238 602C329 602 354 454 354 312C354 172 330 22 240 22C152 22 124 173 124 313S148 602 238 602Z"></path></g><g transform="matrix(.013,0,0,-0.013,61.187,0)"><path d="M241 635C89 635 35 457 35 312C35 153 89 -12 240 -12C390 -12 443 166 443 312C443 466 390 635 241 635ZM238 602C329 602 354 454 354 312C354 172 330 22 240 22C152 22 124 173 124 313S148 602 238 602Z"></path></g><g transform="matrix(.013,0,0,-0.013,67.427,0)"><path d="M241 635C89 635 35 457 35 312C35 153 89 -12 240 -12C390 -12 443 166 443 312C443 466 390 635 241 635ZM238 602C329 602 354 454 354 312C354 172 330 22 240 22C152 22 124 173 124 313S148 602 238 602Z"></path></g><g transform="matrix(.013,0,0,-0.013,73.667,0)"><path d="M384 0V27C293 34 287 42 287 114V635C232 613 172 594 109 583V559L157 557C201 555 205 550 205 499V114C205 42 199 34 109 27V0H384Z"></path></g></svg>;</span> ANOVA with Dunnett’s test applied post hoc).</div>

Cellular Microbiology

fig4

Figure 4

Figure 4: G<sub>1</sub> Cell Cycle Arrest Is Induced by the Fourth Extracellular Loop of Meningococcal PorA in Epithelial and Endothelial Cells 

Cellular Microbiology

G1 Cell Cycle Arrest Is Induced by the Fourth Extracellular Loop of Meningococcal PorA in Epithelial and Endothelial Cells

Figure 4

G₁ Cell Cycle Arrest Is Induced by the Fourth Extracellular Loop of Meningococcal PorA in Epithelial and Endothelial Cells