Segmentation and Morphometric Analysis of Cells from Fluorescence Microscopy Images of Cytoskeletons
Figure 11
Differentiation of intracellular noncytoskeletal regions from the extracellular region. (a) Green regions represent the cytoskeletal pixels, while white ones represent the non-cytoskeletal pixels. The presence of cytoskeletal pixels surrounding (±x, ±y) a non-cytoskeletal pixel of interest was used to distinguish the intracellular non-cytoskeletal region from the extracellular region. If cytoskeletal pixels surrounded the pixel of interest on the x- and y-axes, it was recognized as an intracellular pixel. The yellow and red pixels are recognized as intracellular pixels, although red pixels should be identified as extracellular. (b) The resulting image of this process. Blue regions represent pixels recognized as intracellular as a result of this process. The red arrows show where this process incorrectly labeled some of the extracellular region.
