Structural Equation Modeling for Analyzing Erythrocyte Fatty Acids in Framingham

Algorithm 1

Mplus code for structural equation model M7.

TITLE:

Structural Equation Model M7;

DATA:

FILE IS infile;

VARIABLE:

NAMES ARE C140 C160 C180 C161 C161t C181t C182t C204n6 C224n6 C225n6

   LnC183n3 LnC205n3 C226n3 ID Female;

USEVARIABLES C140 C160 C180 C161 C161t C181t C182t C204n6 C224n6 C225n6

   LnC183n3 LnC205n3 C226n3;

GROUPING is Female (1=Female 0=Male);

AUXILIARY=ID;

ANALYSIS:

TYPE=GENERAL;

ESTIMATOR=MLR;

MODEL:

!MODEL Female;

!Latent variable loadings;

fFISH BY LnC183n3∗  LnC205n3 C226n3 C204n6 C224n6 C225n6;

fSAT BY C226n3∗  C204n6 C140 C160 C180 C161 LnC183n3 C181t C182t;

fTRANS BY C204n6∗  C160 C180 C161t C181t C182t LnC183n3 C140;

!Latent variable means are fixed at 0;

[fFISH@0 fSAT@0 fTRANS@0];

!Fatty acid means are free, or constrained to zero where indicated;

[LnC183n3 LnC205n3 C226n3@0 C224n6 C225n6 C204n6@0 C140 C180 C160@0 C161 C161t C181t@0 C182t];

!Latent variables covariance matrix;

fFISH@1; fSAT@1; fTRANS@1;

fFISH WITH fSAT;

fFISH WITH fTRANS;

fSAT WITH fTRANS;

!Fatty acid residual variances are equal between gender;

C140(1); C160(2); C180(3); C161(4); C161t(5); C181t(6); C182t(7);

C204n6(8); C224n6(9); C225n6(10); LnC205n3(11); C226n3(12); LnC183n3(13);

!Dietary intake covariances are equal between gender;

C140 WITH C160(14); C140 WITH C180(15); C140 WITH C161t(16);

C160 WITH C161(17); C160 WITH C161t(18);

C180 WITH C161(19); C180 WITH C161t(20); C180 WITH C181t(21); C180 WITH C182t(22);

C161 WITH C161t(23);

LnC205n3 WITH C226n3(24);

!Desaturase enzymes covariances are equal between genders;

LnC183n3 WITH LnC205n3(25);

C204n6 WITH LnC205n3(26);

C226n3 WITH C225n6(27);

C226n3 WITH LnC183n3(28);

MODEL male:

!Latent variable loadings;

fFISH BY LnC183n3∗  LnC205n3 C226n3 C204n6 C224n6 C225n6;

fSAT BY C226n3∗  C204n6 C140 C160 C180 C161 LnC183n3 C181t C182t;

fTRANS BY C204n6∗  C160 C180@0 C161t C181t C182t LnC183n3 C140;

!Fatty acid means are free, or constrained to zero where indicated;

[LnC183n3 LnC205n3 C226n3@0 C224n6 C225n6 C204n6@0 C140 C180 C160@0 C161 C161t C181t@0 C182t];

!Fatty acid residual variances are equal between gender;

C140(1); C160(2); C180(3); C161(4); C161t(5); C181t(6); C182t(7);

C204n6(8); C224n6(9); C225n6(10); LnC205n3(11); C226n3(12); LnC183n3(13);

!Dietary intake covariances are equal between gender;

C140 WITH C160(14); C140 WITH C180(15); C140 WITH C161t(16);

C160 WITH C161(17); C160 WITH C161t(18);

C180 WITH C161(19); C180 WITH C161t(20); C180 WITH C181t(21); C180 WITH C182t(22);

C161 WITH C161t(23);

LnC205n3 WITH C226n3(24);

!Desaturase enzymes covariances are equal between genders;

LnC183n3 WITH LnC205n3(25);

C204n6 WITH LnC205n3(26);

C226n3 WITH C225n6(27);

C226n3 WITH LnC183n3(28);

OUTPUT:

SAVEDATA:

FORMAT IS F20.14;

FILE IS outfile;

RESULTS ARE parametersfile;

SAVE=FSCORES;