Image Segmentation and Identification of Paired Antibodies in Breast Tissue
Figure 3
Hematoxylin + DAB staining; sample images and their pseudocolored posterior maps, . The presence of DAB affects the clustering; brown DAB and dark blue regions are grouped into one cluster in the cases above; however, in the general scheme DAB is separated using a trained classifier prior to clustering (see Figure 8). Pseudocolors were assigned as follows: DAB and nuclei appear as blue, stroma appears as green, and lumen/background appears as orange.