Towards Automated Three-Dimensional Tracking of Nephrons through Stacked Histological Image Sets

Figure 8

A manually tracked mouse nephron is shown on the left. The PCT and PST are successfully tracked automatically as shown in the middle plot. Tracking terminates due to diminishing tubule size coupled with artefacts in the inner medulla. A more complete nephron path is obtained with 5 manual corrections on the DTL and 4 on the ATL, as shown on the right plot (semiautomatically). The paths are coloured by the error, or residual, with respect to the manually tracked nephron. The maximum residual (shown as dark red) in this instance is 35 pixels. The black asterisks are points of manual correction. This is acceptable considering that a total of 1222 coordinates make up this path. %; %; %; %.

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