[Retracted] miR-139-5p Inhibits Lung Adenocarcinoma Cell Proliferation, Migration, and Invasion by Targeting MAD2L1

Figure 3

miR-139-5p targeted binds to MAD2L1 and negatively regulates MAD2L1 expression. (a) Venn diagram of target genes of miR-139-5p predicted by miRDB, miDIP, and starBase databases and upregulated DEmRNAs in the TCGA-LUAD; (b) Pearson correlation of miR-139-5p and five candidate genes NPTX1, ELAVL2, FBN2, GPR37, and MAD2L1; (c) Pearson correlation of miR-139-5p and MAD2L1; (d) Box plots of MAD2L1 expression in LUAD tissue and normal tissue in the TCGA-LUAD dataset; (e) Survival curves of patients with high expression of MAD2L1 (red) and low expression of MAD2L1 (blue). The abscissa refers to the time (in years) and the ordinate refers to survival rate; (f) qRT-PCR was used to detect the mRNA expression level of MAD2L1 after transfection of miR-139-5p mimic or miR-139-5p inhibitor; (g) Western blot was employed to examine the protein expression of MAD2L1 after transfection; (h) starBase database was used to predict the binding sites of miR-139-5p on MAD2L1 3UTR; (i) Dual-luciferase reporter gene assay was used for verification of the targeted binding relationship between miR-139-5p and MAD2L1; .

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