[Retracted] The Effect of miR-138 on the Function of Follicular Helper T Cells and the Differentiation of B Cells in Osteosarcoma

Figure 4

The effect of upregulation of miR-138 expression on the immune function of osteosarcoma Tfh cells and the differentiation of B cells. After Tfh cells were transfected with the miR-138 inhibitor (in-miR-138) and its control inhibitor NC (in-NC), (a) qRT-PCR was used to detect the expression of miR-138 in Tfh cells. (b–e) ELISA was used to detect the expression of IL-12 (b), IL-21 (c), INF-γ (d), and IL-10 (e) in the supernatant of Tfh cells. (f–h) Tfh cells were cocultured with B cells, and the expression of IgM (f), IgG (g), and IgA (h) in the supernatant of each group of cells was detected by ELISA. (i) After Tfh cells were cocultured with B cells, the rate of CD27- and CD38-positive cells in B cells was detected by flow cytometry. vs. HC group, ^##vs. in-NC/OS group.

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