[Retracted] Overexpression of PIK3R1 Promotes Bone Formation by Regulating Osteoblast Differentiation and Osteoclast Formation

Figure 4

The impact of PIK3R1 on osteoblast differentiation: (a, b) the transfection efficacy of oe-PIK3R1 determined by qRT-PCR and western blotting; (c) the impact of PIK3R1 upregulation on osteoblast differentiation assessed by ALP and ARS staining; (d) the mRNA levels of osteoblast-related genes assayed via qRT-PCR; (e) the protein levels of osteoblast-related genes measured by western blotting; (f, g) the impact of PIK3R1 upregulation on osteoblast proliferation tested through CCK-8 and EdU; (h) the impact of PIK3R1 upregulation on osteoblast apoptosis measured by flow cytometry; .

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