PAR2 Promoter Hypomethylation Regulates PAR2 Gene Expression and Promotes Lung Adenocarcinoma Cell Progression

Figure 1

Bioinformatics analysis suggests that PAR2 promoter methylation is related to PAR2 mRNA expression and the prognosis of LUAD patients. (a) “MethylMix” package was used to screen methylation-driven genes from TCGA-LUAD dataset as plotted in a heat map, with red representing high methylation and green representing low methylation. PAR2 was identified and its (b) promoter methylation level was detected in tumor samples (histogram) and normal tissue samples (horizontal black line) in TCGA-LUAD dataset. (c) Correlation analysis for PAR2 promoter methylation and gene expression. (d) Relative expression level of PAR2 in TCGA was examined. (e) Survival analysis shows overall survival difference between high/low PAR2 promoter methylation level in LUAD patients (red line: hypermethylation; blue line: hypomethylation). (f) Overall survival difference in LUAD patients between hyper- and low-expression (red line) and hypo- and high-expression (blue line) groups. Afterwards, (g) the promoter methylation level of PAR2 was determined in different tumor stages and T stages combined with clinical information. (h) Correlation analysis between methylation level of three PAR2-related methylated sites and PAR2 expression level, respectively.

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