[Retracted] miR-223-5p Suppresses OTX1 to Mediate Malignant Progression of Lung Squamous Cell Carcinoma Cells

Figure 3

miR-223-5p targets and inhibits OTX1 expression: (a) volcano plot of DEmRNAs in TCGA-LUSC; (b) intersection between targeted genes of miR-223-5p predicted by TargetScan and miRTarBase databases and the upregulated DEmRNAs in TCGA-LUSC dataset; (c) box plot of OTX1 expression in tumor and normal samples in TCGA; (d) correlation between miR-223-5p and 19 candidate targeted genes; (e) predicted binding sites between miR-223-5p and OTX1; (f) the targeted binding between miR-223-5p and OTX1 was identified by dual-luciferase assay; (g) OTX1 mRNA expression in cells overexpressing miR-223-5p or inhibiting miR-223-5p was detected by qRT-PCR; (h) OTX1 protein expression in cells overexpressing miR-223-5p or inhibiting miR-223-5p was assessed by Western blot; compare miR-223-5p mimic+OTX1-WT with mimic-NC+OTX1-WT, compare miR-223-5p mimic with mimic NC, and compare miR-223-5p inhibitor with inhibitor NC, .

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