[Retracted] miR-223-5p Suppresses OTX1 to Mediate Malignant Progression of Lung Squamous Cell Carcinoma Cells

Figure 4

OTX1 induces JAK/STAT signaling pathway activation and potentiates the proliferation, invasion, and migration of LUSC cells. (a) The signaling pathway regulated by OTX1 was analyzed via GSEA. (b) Relative expression of the proteins relevant to the JAK/STAT signaling pathway after OTX1 was overexpressed or silenced was detected by Western blot. (c) OTX1 mRNA expression in SK-MES-1 after OTX1 was overexpressed or silenced. (d) Changes of SK-MES-1 cell proliferation upon overexpression or silencing of OTX1 were detected by MTT assay. (e) Changes of SK-MES-1 cell invasion upon overexpression or silencing of OTX1 were detected by Transwell (×100). (f) Changes of SK-MES-1 cell migration upon overexpression or silencing of OTX1 were detected by wound healing assay (×40); means as the oe-OTX1 group versus oe-NC, or si-OTX1 versus si-NC.

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