[Retracted] Inhibition of miR-15a-5p Promotes the Chemoresistance to Pirarubicin in Hepatocellular Carcinoma via Targeting eIF4E

Figure 4

eIF4E mediates the effects of miR-15a-5p on the THP chemoresistance of HepG2 cells. (a) qRT-PCR and western blot assays were performed to evaluate the miR-15a-5p and eIF4E protein expressions in THP-treated HepG2 cells transfected with miR-15a-5p mimic or pc-eIF4E. (b) The viability and proliferation of THP-treated HepG2 cells transfected with miR-15a-5p mimic or pc-eIF4E were assessed by CCK-8 and EdU assays. (c) The apoptosis of THP-treated HepG2 cells transfected with miR-15a-5p mimic or pc-eIF4E was evaluated by flow cytometer. (d) The caspase-3 protein level in THP-treated HepG2 cells transfected with miR-15a-5p mimic or pc-eIF4E was evaluated by western blot assay. and vs. the HepG2 group; ^# and ^## vs. the HepG2+THP+miR-NC+pc-NC group; ^&& vs. the HepG2+THP+miR-15a-5p mimic+pc-NC group. All data were presented as . .

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