Naloxone Protects against Lipopolysaccharide-Induced Neuroinflammation and Microglial Activation via Inhibiting ATP-Sensitive Potassium Channel

Figure 2

Effects of naloxone on the inflammatory responses and microglial activation in LPS-induced BV-2 cells. (a) The release of NO was measured by Griess’s kit. (b–d) The expressions of proinflammatory cytokines TNF-α, IL-1β, and IL-6 were measured by ELISA. (e) The expressions of proinflammatory enzymes iNOS and COX-2 were determined by western blot. (f) The expression of Iba-1 in LPS-induced BV-2 cells was evaluated by immunofluoresence. ; .

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