[Retracted] Circular RNA hsa_circ_0001658 Inhibits Intervertebral Disc Degeneration Development by Regulating hsa-miR-181c-5p/FAS

Figure 2

hsa-miR-181c-5p silence or hsa_circ_0001658 overexpression inhibited the proliferation and promoted apoptosis of hNPCs. (a) The binding sites in hsa_circ_0001658 and hsa-miR-181c-5p predicted by starBase. (b) The hsa-miR-181c-5p level in hNPCs (control), 5 ng/ml TNF-α- and IL-1β-treated hNPCs, 5 ng/ml TNF-α- and IL-1β-treated hNPCs combined with hsa-miR-181c-5p mimic transfection, and 5 ng/ml TNF-α- and IL-1β-treated hNPCs combined with si-hsa_circ_0001658 transfection was detected by qRT-PCR. (c) The proliferation ability of 5 ng/ml TNF-α- and IL-1β-treated hNPCs, 5 ng/ml TNF-α- and IL-1β-treated hNPCs transfected with hsa-miR-181c-5p inhibitor, and 5 ng/ml TNF-α- and IL-1β-treated hNPCs combined with hsa_circ_0001658 transfection was detected by Cell Counting Kit-8 (CCK-8). (d) The apoptosis rates of 5 ng/ml TNF-α- and IL-1β-treated hNPCs, 5 ng/ml TNF-α- and IL-1β-treated hNPCs transfected with hsa-miR-181c-5p inhibitor, and 5 ng/ml TNF-α- and IL-1β-treated hNPCs combined with hsa_circ_0001658 transfection were detected by flow cytometry. (e) The expression level of MMP-3, MMP-13, collagen II, and aggrecan in 5 ng/ml TNF-α- and IL-1β-treated hNPCs, 5 ng/ml TNF-α- and IL-1β-treated hNPCs transfected with hsa-miR-181c-5p inhibitor, and 5 ng/ml TNF-α- and IL-1β-treated hNPCs combined with hsa_circ_0001658 transfection was detected by western blot. , , and compared with control.

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