[Retracted] Circular RNA hsa_circ_0001658 Inhibits Intervertebral Disc Degeneration Development by Regulating hsa-miR-181c-5p/FAS

Figure 3

hsa_circ_0001658 acted as a sponge of hsa-miR-181c-5p. (a) The correlation between the expression levels of hsa-miR-181c-5p and hsa_circ_0001658 in NP tissue cells of IDD patients. (b, c) Relative luciferase activity was conducted after hsa_circ_0001658 WT1, hsa_circ_0001658 WT2, hsa_circ_0001658 Mut1, hsa_circ_0001658 Mut2, and hsa-miR-181c-5p mimic cotransfection into hNPCs. (d) The relative expression level of hsa_circ_0001658 in hNPCs with no template control (NC), hsa_circ_0001658 overexpression vector transfection was detected by qRT-PCR. (e) The relative expression level of hsa_circ_0001658 in NP tissues of IDD patients (data from the GSE67566 dataset). (f) The cell proliferation of NC, hsa_circ_0001658, and hsa_circ_0001658+hsa-miR-181c-5p mimic-cotransfected hNPCs was detected by Cell Counting Kit-8 (CCK-8). (g) The apoptotic rate of NC, hsa_circ_0001658, and hsa_circ_0001658+hsa-miR-181c-5p mimic-cotransfected hNPCs was detected by flow cytometry. , , , and .

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