[Retracted] Circular RNA hsa_circ_0001658 Inhibits Intervertebral Disc Degeneration Development by Regulating hsa-miR-181c-5p/FAS

Figure 5

hsa_circ_0001658 functioned in hNPCs through targeting hsa-miR-181c-5p/FAS. (a) hsa_circ_0001658 relative expression level in hNPCs for no template control (si-NC), hsa_circ_0001658 overexpression vector, and hsa_circ_0001658 siRNA transfection was detected by qRT-PCR. (b) The relative expression level of FAS protein in hNPCs for NC, hsa_circ_0001658 overexpression vector, and hsa_circ_0001658+ hsa-miR-181c-5p mimic transfection was detected by western blot. (c) The relative expression levels of hsa_circ_0001658 in hNPCs (control), 5 ng/ml TNF-α- and IL-1β-treated hNPCs (NC), and 5 ng/ml TNF-α- and IL-1β-treated hNPCs for hsa_circ_0001658 siRNA transfection were detected by qRT-PCR. (d) The relative FAS protein expression level of control, NC, and 5 ng/ml TNF-α- and IL-1β-treated hNPCs for hsa_circ_0001658 siRNA transfection was detected by western blot. (e) The cell proliferation of 5 ng/ml TNF-α- and IL-1β-treated hNPCs for no temple control (NC), si-hsa_circ_0001658, and FAS short hairpin RNA (sh-FAS) transfection was detected by Cell Counting Kit-8 (CCK-8) assay. (f) The cell apoptosis rate of 5 ng/ml TNF-α- and IL-1β-treated hNPCs for NC, si-hsa_circ_0001658, and sh-FAS transfection was detected by flow cytometry. , , , and .

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