[Retracted] lncRNA MSC-AS1/miRNA-429 Axis Mediates Growth and Metastasis of Nasopharyngeal Carcinoma via JAK1/STAT3 Signaling Pathway

Figure 2

Effect of abnormal MSC-AS1 effect on C666-1 cell proliferation, migration, and invasion. (a) The transfection efficiency was tested by qRT-PCR analysis. The expression of MSC-AS1 was remarkably accumulated in the pcDNA-MSC-AS1 group and significantly declined in the si-MSC-AS1 group compared with controls. , , and . (b) The cell viability changes disturbed by MSC-AS1 dysregulation were detected by MTT assay. Cell viability was significantly reduced in the si-MSC-AS1 group. , , and . (c) cell proliferation was observed by cell colony formation assay. The colony formation ability was significantly elevated in the pcDNA-MSC-AS1 group and obviously decreased in the si-MSC-AS1 group. NS: no significant difference; . Cell wound scratch assay (d) and transwell invasion assay (e) were performed to observe the changes in migration and invasion of C666-1 cells induced by MSC-AS1 deregulation. The cell migration and invasion were all significantly enhanced in the pcDNA-MSC-AS1 group and reduced in the si-MSC-AS1 group. NS: no significant difference; ; .

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