[Retracted] lncRNA MSC-AS1/miRNA-429 Axis Mediates Growth and Metastasis of Nasopharyngeal Carcinoma via JAK1/STAT3 Signaling Pathway

Figure 3

The relationship between MSC-AS1 and miR-429. (a) The binding site between MSC-AS1 and miR-429 was predicted by starBase, and the mutant binding sequence was designed. (b) Double luciferase report assay was performed to validate the target interaction between MSC-AS1 and miR-429. Results determined that miR-429 was the direct target for MSC-AS1. . (c) RNA pull down analysis was conducted to further validate the interaction between MSC-AS1 and miR-429. miR-429 could be pulled down by Bio-MSC-AS1-pellets complex. NS: no significant difference; . (d) The interaction between MSC-AS1 and miR-429 was detected by qRT-PCR analysis after perturbed MSC-AS1 expression. Results showed that the expression of miR-429 was obviously reduced in MSC-AS1 overexpression cells, while significantly elevated after MSC-AS1 silence. NS: no significant difference; .

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