[Retracted] lncRNA MSC-AS1/miRNA-429 Axis Mediates Growth and Metastasis of Nasopharyngeal Carcinoma via JAK1/STAT3 Signaling Pathway

Figure 4

The carcinogenic effect of MSC-AS1 on C666-1 cells is disturbed by intervening miR-429 expression. (a) After the C666-1 cells were cotransfected with si-MSC-AS1 and miR-429 inhibitor, the expression of miR-429 was measured by qRT-PCR analysis. The increased miR-429 expression induced by MSC-AS1 silence was repressed by the miR-429 inhibitor. NS: no significant difference; ; . (b) Cell viability after cotransfected with si-MSC-AS1 and miR-429 inhibitor was estimated by MTT assay. Cell viability was significantly declined by MSC-AS1 expression inhibition, which was abolished by cotransfected with miR-429 inhibitor. , compared with controls. (c) Colony formation assay was performed to analyze the cell proliferation. The number of cell colonies was significantly decreased after MSC-AS1 knockdown and was reversely increased after cotransfected with miRNA-429 inhibitor. NS: no significant difference; . (d) Cell migration ability was observed by wound scratch. Cell migration was obviously suppressed in MSC-AS1 knockdown cells and was reversed by cotransfection with miRNA-429 inhibitor. NS: no significant difference; . (e) Cell invasion ability was evaluated by transwell assay. Cell invasion ability was remarkably inhibited in cells transfected with si- MSC-AS1 + miRNA-ctrl but was obviously enhanced in the si-MSC-AS1 + miR-429 inhibitor group. NS: no significant difference; ; .

(a)

(b)

(c)

(d)

(e)