[Retracted] Analysis of the Role and Mechanism of ZEB1 in Regulating Cervical Carcinoma Progression via Modulating PD-1/PD-L1 Checkpoint

Figure 3

ZEB1 transcription up-regulates the expression of PD-1/PD-L1 and promotes EMT. (a) We further studied the specific regulation of ZEB1 on PD-1/PD-L1 in cervical carcinoma and found through JASPAR (http://jaspar.genereg.net/) that ZEB1 has two binding sites on the PD-L1 promoter. (b–d) The mRNA and protein expression of ZEB1 and PD-L1 after ZEB1 knockdown was detected by RT-PCR and Western blotting experiments. (e) ChIP assay was used to analyze the DNA fragment of PD-L1 promoter in the precipitation of ZEB1 antibody. (f) The dual-luciferase assay was used to detect the effect of ZEB1 knockdown on the luciferase activity of PD-L1 promoter. ; indicates , indicates P <0.01, and indicates compared between two groups.

(a)

(b)

(c)

(d)

(e)

(f)