[Retracted] +HOXA10-AS Promotes Malignant Phenotypes of Gastric Cancer via Upregulating HOXA10

Figure 5

HOXA10-AS accelerated malignant phenotypes of GC cells by promoting HOXA10 expression. HGC27 and AGS cells were transfected with empty vector (vector), HOXA10-AS-overexpressing vector (HOXA10-AS) or HOXA10-AS in combination with si-HOXA10. (a) HOXA10-AS expression was examined by qRT-PCR (). (b) Western blot analysis of HOXA10 (). (c) Cell proliferation was determined with CCK-8 assay (). (d) EdU incorporation (red) in HGC27 and AGS cells (; scale bar, 100 μm). The nucleus was stained with DAPI (blue). (e) Flow cytometry analysis of cell apoptosis (). (f) The wound healing assay was applied to examine cell migration (; scale bar, 100 μm). (g) The invasive capacity of HGC27 and AGS cells were examined using the transwell assay (; scale bar, 50 μm).

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