[Retracted] Erchen Decoction Ameliorates the Metabolic Abnormalities of High-Fat Diet-Fed Rats

Figure 4

ECD increased the expression levels of UCP-1 and PGC-1α in interscapular brown adipose tissue in HFD-fed rats. (a) ECD increased UCP-1 and PGC-1α mRNA expression levels in interscapular brown adipose tissue in HFD-fed rats. UCP-1/GAPDH mRNA and PGC-1α/GAPDH mRNA levels were measured by qPCR. Expression in the CON group was normalized to 1, . (b) Representative immunohistochemical analysis showing the expression of UCP-1 in interscapular brown adipose tissue cross-sections. Bar scale 200 μm. (c) ECD increased UCP-1 and PGC-1α protein expression levels in interscapular brown adipose tissue in HFD-fed rats. UCP-1 and PGC-1α densitometry values were adjusted to GAPDH intensity, then normalized to expression from the control sample. Expression in the CON group was normalized to 1, . ECD: Erchen decoction; CON: control group; HF: high-fat diet group; ECDL: high-fat diet and low dose of ECD treatment group; ECDH: high-fat diet and high dose of ECD treatment group. versus CON; ^# versus HF.

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