[Retracted] miR-29b Regulates Lung Cancer Progression by Downregulating FEM1B and Inhibiting the FOX01/AKT Pathway

Figure 3

FEM1B is a miR-29b regulatory target gene. (a) The predicted miR-29b binding site in the 3-UTR of FEM1B and the mutated 3-UTR of FEM1B, which were generated using the complementary sequence in the seed region of miR-29b. (b) miR-29b or NC and a luciferase vector encoding the WT or MUT FEM1B 3-UTR were transfected into 293T cells, and the relative luciferase activity was measured. (c) Overexpression of miR-29b inhibited the expression of FEM1B mRNA in NSCLC cells. (d) Overexpression of miR-29b inhibited the expression of FEM1B protein in NSCLC cells; compared with the NC-A549 group; ^# compared with the NC-NCI-H-1792 group.

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