[Retracted] Double-Negative T Cells Attenuate Cisplatin-Induced Acute Kidney Injury via Upregulating IL-10/AT2R Axis

Figure 4

Upregulation of AT2R by adding IL-10 or DN T cells ameliorates cisplatin-induced AKI. The effect of DN T cells or IL-10 on AT2R expression in HK-2 cells pretreated with cisplatin was detected by (a) qPCR and (b) Western blot. Whole-cell lysates from HK-2 cells cotreated with cisplatin and DN T cells or IL-10 were immunoblotted with anti-AT2R antibody. GAPDH was used as a control. (c) CCK-8 assay and (d, e) flow cytometry of HK-2 cells pretreated with cisplatin, PD123319, and DN T cells or IL-10; HK-2 cells were pretreated with cisplatin and DN T cells or IL-10, and then, PD123319 was added into the culture. (d) HK-2 cells were treated by the same strategy, and the KIM, calbindin, TIMP-1, and Bcl-2 protein levels of HK-2 were immunoblotted with anti-KIM, anti-calbindin, anti-TIMP-1, and anti-Bcl-2 antibodies; GAPDH was used as an internal control. Statistical significance was defined as , , and .

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