Chelerythrine Chloride Inhibits Stemness of Melanoma Cancer Stem-Like Cells (CSCs) Potentially via Inducing Reactive Oxygen Species and Causing Mitochondria Dysfunction

Figure 2

Melanoma CSCs were sensitive to CHE. (a) 5-40 μmol/L of CHE was cocultured with A2058 and A375 CSCs or PCs for 24 h, and then, cell viability was measured by CCK-8 assay. (b) IC30 of CHE to cells were calculated. vs. PC group (). (c) After 30 or 40 μmol/L CHE treatment for 24 h, cell apoptosis was measured by performing Annexin V-FITC/PI double staining followed by flow cytometry assay. vs. PC group ().

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