Preliminary Investigation on the Relationship between Raman Spectra of Beef and Metmyoglobin and Metmyoglobin Reductase Activity

Table 3

Raman modes useful in interpretation of the protein structure adapted from the works of Li-Chan [19] and Beattie et al. [3].

Origin	Wavenumber (cm^−1)	Assignment	Structural information obtained
Phenylalanine	1006	Breathing ring	Conformation insensitive; useful as an internal intensity standard
Histidine	1409	N-Diimidazole	Probe of ionization state, metalloprotein structure, and proton transfer in deuterated solution
Tryptophan	760, 880, 1360	Indole ring	Sharp intense band indicates buried residues; sensitive to environment polarity
Aspartic acid, glutamic acid	1400–1430	C=O stretch of COO−	Ionized carboxyl groups
Amide III	1230–1240	N-H in-plane bend, C-N stretch	Antiparallel g-sheet
Amide I	1650–1660	Amide C=O stretch, N-H wag	α-Helix
Aliphatic amino acids	1450, 1465	C-H bending	Microenvironment, polarity