[Retracted] Low-Dose X-Ray Increases Paracellular Permeability of Human Renal Glomerular Endothelial Cells

Figure 3

X-ray irradiation increases the paracellular permeability of HRGECs through the disruption of cell junctions. HRGECs was cultured for seven days under 0, 0.1, 0.5, 1.0, and 2.0 Gy X-ray irradiation. 50 kDa FITC-dextran was used to determine the permeability of HRDECs (a). The transfer amount of 50 kDa FITC-dextran across the HRGECs monolayer without X-ray irradiation was set to 1. Western blot bands of CLDN5 (b) and VE-cadherin (d) were tested and analyzed. GAPDH was used as an internal control. The mRNA results of CLDN5 (c) and VE-cadherin (e) were detected by qRT-PCR. Immunofluorescence images of cell junctions (f) were shown by staining VE-cadherin with fluorescent antibodies (green), and DAPI was used for nuclear staining (blue). ; .

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