[Retracted] Melatonin Attenuates Cisplatin-Induced Ototoxicity via Regulating the Cell Apoptosis of the Inner Ear

Figure 1

Melatonin significantly attenuates cisplatin-induced hearing loss. (a) Comparison of frequency hearing thresholds of mice in each group after cisplatin and melatonin pretreatment. The cisplatin group has significantly higher thresholds in all frequencies than the control group. Melatonin pretreatment attenuated the cisplatin-induced hearing loss. In addition, the control group and the melatonin group do not have any significant difference (, ). (b) Hematoxylin-eosin staining showed the Corti’s morphological change of the four groups. In the cisplatin group, the outer hair cells were obviously missing, the inner hair cells had abnormal morphology, and the basement membrane became shrunk. Pretreatment of melatonin could reduce this damage (200×). (c) Basement membrane phalloidin staining showed the loss of outer hair cells after cisplatin treatment and melatonin pretreatment. The hair cells were stained using phalloidin (green), the nuclei were stained with DAPI (blue). Melatonin pretreatment can reduce hair cell loss caused by cisplatin, .

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