[Retracted] circ_0041795 Induces YAP1 Upregulation to Accelerate the Progression of Diabetic Retinopathy through Binding to miR-589-5p

Figure 5

miR-589-5p targeted YAP1 to suppress HG-induced cell dysfunction. ARPE-19 cells were performed with treatment of NG, HG, HG+miR-NC, HG+miR-589-5p, HG+miR-589-5p+pcDNA, or HG+miR-589-5p+YAP1. (a) YAP1 protein detection was performed using western blot. (b) Cell viability analysis was performed using CCK-8 assay. (c) Cell proliferation determination was performed using EdU assay. (d, e) The assessment of cell apoptosis was performed using flow cytometry. (f) The protein examination of PCNA and Bax was performed using western blot. (g, h) The inflammatory cytokines were detected using ELISA. (i, j) Oxidative stress was evaluated using SOD activity and MDA level. Two-way ANOVA was used in (f), and one-way ANOVA was used in other graphs. , , , and .

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