[Retracted] miR-135a-5p Suppresses TBK1 and Activates NRF2/TXNIP Antioxidant Pathway in LPS-Driven ALI in Mice

Figure 1

Overexpressed miR-135a-5p or inhibited TBK1 attenuates LPS-driven ALI and inflammatory response in mice. (a) Representative histopathological examination of mouse lung tissues by HE staining in LPS-driven group, LPS-driven group transfected with miR-135a-5p mimics, LPS-driven group transfected with si-TBK1, and corresponding NCs and the healthy controls. Scale bars: 25 μm, magnified 400x, for each group. (b) Expression of miR-135a-5p by qRT-PCR. (c) mRNA of TBK1 was determined through RT-qPCR, and TBK1 proteomic content was evaluated using Western blotting. (d) IL-1β, IL-6, TNF-α, and IL-10 levels were determined through ELISA kits. values were assessed through Student’s test.

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