[Retracted] miR-135a-5p Suppresses TBK1 and Activates NRF2/TXNIP Antioxidant Pathway in LPS-Driven ALI in Mice

Figure 3

miR-135a-5p mediates inflammation and oxidative stress through regulating TBK1 in LPS-driven murine ALI. (a) mRNAs for miR-135a-5p and TBK1 were determined through RT-qPCR, and protein level for TBK1 was evaluated using Western blotting in lung tissues in LPS-driven group, LPS-driven group transfected with miR-135a-5p mimics, LPS-driven group transfected with miR-135a-5p mimics and pcDNA3.1-TBK1, and the corresponding NC controls. (b) Levels of IL-1β, IL-6, TNF-α, and IL-10 were determined using ELISA kits. (c) Protein levels of NRF2 and TXNIP were detected using Western blotting. (d) MDA, ROS, and SOD of LPS-driven lung tissues were analyzed using ELISA kits. values were assessed through Student’s test.

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