[Retracted] miR-135a-5p Suppresses TBK1 and Activates NRF2/TXNIP Antioxidant Pathway in LPS-Driven ALI in Mice

Figure 4

miR-135a-5p negatively regulates TBK1 expression. (a) miR-135a-5p transfected efficiency was analyzed using qRT-PCR. (b) Biding site between miR-135a-5p and TBK1 was predicted by Starbase (http://starbase.sysu.edu.cn/) and TargetScan (http://www.Targetscan.org/). (c) Targeting relationship between miR-135a-5p and TBK1 was analyzed by dual-luciferase reporter assay. (d, e) mRNA and protein levels of TBK1 were detected using qRT-PCR and Western blotting. values were assessed through Student’s test.

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