[Retracted] miR-135a-5p Suppresses TBK1 and Activates NRF2/TXNIP Antioxidant Pathway in LPS-Driven ALI in Mice

Figure 5

miR-135a-5p mediates inflammation and oxidative stress through regulating TBK1 in LPS-driven MLE-12 cells. (a) Transfection efficiency of TBK1 was confirmed using qRT-PCR. (b) Expression of miR-135a-5p by qRT-PCR. (c) mRNA and protein levels of TBK1 were detected using qRT-PCR or Western blotting in LPS-driven MLE-12 cells, LPS-driven MLE-12 cells transfected with miR-135a-5p mimics, LPS-driven MLE-12 cells transfected with miR-135a-5p mimics and pcDNA3.1-TBK1, and the corresponding NC controls. (d) Serums IL-1β, IL-6, TNF-α, and IL-10 were determined using ELISA kits. (e) Supernatant protein levels of NRF2 and TXNIP were detected using Western blotting. (f) Supernatant contents of MDA, ROS, and SOD were determined using ELISA kits. values were assessed through Student’s test.

(a)

(b)

(c)

(d)

(e)

(f)