[Retracted] lncRNA NBAT1 Inhibits Cell Metastasis and Promotes Apoptosis in Endometrial Cancer by Sponging miR-21-5p to Regulate PTEN

Figure 5

lncRNA NBTA1 elevates PTEN expression via sponging miR-21 in EC cells. (a) The complementary sequence of miR-21-5p in PTEN transcripts. (b) The binding ability between PTEN and miR-21-5p in HEC-1A cells was assessed using a luciferase reporter assay. (c) The relative mRNA expression of PTEN in EC and adjacent normal tissues was assessed using RT-qPCR. (d) The protein expression of PTEN in normal human ESC and EC cell lines was detected using Western blot. (e) The protein expression of PTEN in HEC-1A cells transfected with NBAT1 or/and miR-21-5p mimics was detected by Western blot. (f) The protein expression level of PTEN in Ishikawa cells transfected with NBAT1 or/and miR-21-5p mimics was detected by Western blot. vs. adjacent tissues; vs. miR-NC.

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