CREB1 Transcriptionally Activates LTBR to Promote the NF-κB Pathway and Apoptosis in Lung Epithelial Cells

Figure 1

LTBR and CREB1 are upregulated in lungs of hyperoxia-stimulated mice and in hyperoxia-treated A549 and ATII cells. (a) Body weight of mice (in normoxia group and hyperoxia group) at postnatal day 14. (b) Radial alveolar count of mice. (c) Median alveolar septal wall thickness of mice. (d) Relative LTBR and CREB1 expression in lungs of hyperoxia-stimulated mouse model of BPD. PCR analysis was used. (e, f) LTBR and CREB1 proteins in lungs of BPD mice. Western blotting was used and relative grey density of LTBR was calculated using the ImageJ software. (g) Pearson correlation analysis was used to reveal expression correlation of LTBR and CREB1 in lung tissues of hyperoxia-stimulated mice. (h) Relative LTBR and CREB1 expression in A549 cells after exposure to 60% O₂ for 24 h and 48 h. PCR analysis was conducted. (i) Relative LTBR and CREB1 expression in A549 cells after exposure to 21%, 40%, 60%, and 85% O₂ for 48 h. PCR analysis was used. (j) Relative LTBR and CREB1 expression in ATII cells after exposure to 60% O₂ for 24 h and 48 h. PCR analysis was performed. (k) Relative LTBR and CREB1 expression in ATII cells after exposure to 21%, 40%, 60%, and 85% O₂ for 48 h. PCR analysis was used. , , .

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