CREB1 Transcriptionally Activates LTBR to Promote the NF-κB Pathway and Apoptosis in Lung Epithelial Cells

Figure 6

Si-LTBR rescues the effects of pcDNA-CREB1 in the viability, apoptosis, and NF-κB pathway-associated proteins in hyperoxia-stimulated ATII cells. (a) Cell viability of hyperoxia-treated A549 and ATII cells after transfection of pcDNA-NC, pcDNA-CREB1, and cotransfection of pcDNA-CREB1 + si-LTBR. (b, c) Flow cytometry results in A549 and ATII cells after indicated transfections. (d) Quantification analysis of the flow cytometry results in b and c to show cell apoptosis rate. (e) The ratio of p-IKKα/IKKα and relative grey density of p52 and RELB in hyperoxia-stimulated A549 and ATII cells were measured by western blotting. , .

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