DAB2IP Stabilizes p27^Kip1 via Suppressing PI3K/AKT Signaling in Clear Cell Renal Cell Carcinoma

Figure 4

Loss of DAB2IP promotes p27 degradation. (a) Real-time PCR detecting p27 mRNA expression in Con and KD cells. ns: not significant. (b) Western blotting of p27 expression in Con and KD cells with or without MG132 treatment (10 μM, 9 h). (c) Con and KD cells were pretreated with serum-free medium for 2 h, followed by the CHX (7.5 μg/ml) treatment. After the indicated time of CHX treatment, the expression of p27 in cells was determined by Western blotting. Quantification of p27 protein levels were shown. (d) IP detecting of the ubiquitin-binding p27 (Ub) in 786-O Con and KD cells.

(a)

(b)

(c)

(d)