DAB2IP Stabilizes p27^Kip1 via Suppressing PI3K/AKT Signaling in Clear Cell Renal Cell Carcinoma

Figure 6

AKT mediates the regulation of p27 by DAB2IP. (a) Western blotting of total and phosphorylated AKT in Con and KD cells. (b) Caki-2KD and 786-O KD cells were treated with PI3K/AKT pathway inhibitor LY294002 for 24 h, and then, total and phosphorylated AKT and p27 were determined. (c) 786-O KD cells were either transfected with VC, DAB2IP full-length cDNA (F) or truncate cDNA coding the CPR domain of DAB2IP (CPR), and then, Flag-tag antibody was used to detect the exogenous DAB2IP expression, and the expression of AKT and p27 in cells was determined by Western blotting. 786-O Con cells were either transfected with VC, constitutively active AKT (AKT CA), WT or mutant (S10A) p27 constructs, and then, (d) protein expression and (e) cell viability in the indicated cells were determined. .

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