NGS Analysis of Clonality and Minimal Residual Disease in a Patient with Concurrent Richter’s Transformation and CLL/SLL

Table 1

Identification of a clonal population in the lymph node and bone marrow.

	Total reads	Clonal (%)	LQ (%)	PCB (%)
Lymph node
FR1-J	29,955	41.81	40.96	0.92
FR2-J	83,646	47.94	26.23	0.25
FR3-J	241,759	81.25	12.56	0.43
Diagnostic bone marrow
FR1-J	365,953	87.83	1.46	0.07
FR2-J	183,447	67.18	1.83	0.40
FR3-J	292,806	82.76	2.24	0.42

Total reads, % clonal, % LymphoQuant (LQ), and % polyclonal background (PCB) reads from FR1-J, FR2-J, and FR3-J primer sets are shown. The clonal VDJ rearrangement was V1-202/D5-1201/J402. The LQ rearrangement was V1-6901/D6-1301/J603.