Case Report

NGS Analysis of Clonality and Minimal Residual Disease in a Patient with Concurrent Richter’s Transformation and CLL/SLL

Table 1

Identification of a clonal population in the lymph node and bone marrow.

Total readsClonal (%)LQ (%)PCB (%)

Lymph node
FR1-J29,95541.8140.960.92
FR2-J83,64647.9426.230.25
FR3-J241,75981.2512.560.43

Diagnostic bone marrow
FR1-J365,95387.831.460.07
FR2-J183,44767.181.830.40
FR3-J292,80682.762.240.42

Total reads, % clonal, % LymphoQuant (LQ), and % polyclonal background (PCB) reads from FR1-J, FR2-J, and FR3-J primer sets are shown. The clonal VDJ rearrangement was V1-202/D5-1201/J402. The LQ rearrangement was V1-6901/D6-1301/J603.