An Immunocompetent HIV-Negative Elderly Patient with Low-Grade Fever, Generalized Lymphadenopathy, Splenomegaly, and Acute Phase Response: Do Not Forget Castleman Disease

Figure 3

HHV-8 immunohistochemical stain, original magnification ×40. Cells with plasmablastic morphology are HHV8-infected cells (brown nuclei). These cells were polytypic (immunostaining for kappa and lambda light chains (data not shown)). In order to exclude the possibility of random positive counting, the immunostaining section was split into four fields and cells positive for HHV8 were counted in each of these four quartiles. The measurements were grouped for each quartile. Pairwise comparisons among groups using the student t test showed values >0.05 in all cases. In addition, repeating procedures concerning immunohistochemical staining for HHV8 were performed in other two different histological sections producing finally similar results.