Association of CYP2C9∗3 and CYP2C8∗3 Non-Functional Alleles with Ibuprofen-Induced Upper Gastrointestinal Toxicity in a Saudi Patient

Figure 1

Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) results of CYP2C9∗3 and CYP2C8∗3 polymorphisms. (a) Agarose gel electrophoresis analysis for CYP2C9∗3 polymorphism following digestion with Mph1103I (NsiI). Lane 1: 100 bp DNA marker. Lane 2: undigested PCR product (168 bp). Lane 3: digestion of the homozygous mutant CC genotype yields two bands (50 and 118 bp). Since the molecular weight of 50 bp is very small, it could not be distinguished on the gel. The major visible band was of 118 bp. (b) Agarose gel electrophoresis analysis for CYP2C8∗3 polymorphism following digestion with PdmI (XmnI). Lane 1: 50 bp DNA marker. Lane 2: undigested PCR product (117 bp). Lane 3: digestion of the homozygous mutant GG genotype is not cut and yields one band (117 bp).

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