The Partial Duplication of the 5′ Segment of KMT2A Revealed KMT2A-MLLT10 Rearrangement in a Boy with Acute Myeloid Leukemia

Figure 1

FISH and RT-PCR of the KMT2A rearrangement. (a) FISH of the KMT2A break apart signal: the green signal shows the 5′ segment, and the red signal shows the 3′ segment of KMT2A. FISH revealed 2 normal signals (arrowhead) and 1 additional 5′ segment (arrow). Positive cells with duplication of the 5′ segment of KMT2 were observed in 686 out of 1000 cells. (b) The characterization of KMT2A-MLLT10 fusion transcript at bone marrow by RT-PCR. 1: ladder; 2: normal control; 3: at diagnosis; 4: after induction therapy 1; 5: after induction therapy 2; 6: after intensification 1; 7: after intensification 2; 8 and 9: after intensification 3.

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