Increased Levels of ER Stress and Apoptosis in a Sheep Model for Pulmonary Fibrosis Are Alleviated by In Vivo Blockade of the KCa3.1 Ion Channel

Figure 2

Click-iT™ TUNEL Colorimetric assay to identify apoptotic type II AECs and macrophages in sheep parenchymal lung tissues. (a) A representative photomicrograph, captured under 40x magnification for the evaluation. Arrows indicate apoptotic type II alveolar epithelial cells (AECs) and macrophages (M). Graphs represent the number of apoptotic type II AECs and macrophages between bleomycin and saline infusion at the fibrotic stage in sheep lung tissues (n = 8) (b, c). Each bar represents the mean ± standard error of the mean. The tissues were sampled from lung segments at post mortem as indicated in Figures 1(a) and 1(b). Significance was determined by t-test. and .

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