Increased Proportion of Dual-Positive Th2–Th17 Cells Promotes a More Severe Subtype of Asthma

Figure 4

A more severe asthma subtype caused by dual-positive Th2–Th17 cell adoption. Mice received dual-positive Th2–Th17 cells obtained from asthma/differentiation mice (adoption/asthma group) or naïve CD4⁺ T cells (asthma group) separately and then received allergens to build an asthma model. Twenty-four hours after the last challenge, (a) lung resistance was assessed by a Buxco instrument in response to methacholine (Mch). (b) Total number, eosinophil, neutrophil, lymphocyte, and macrophage cells in bronchoalveolar lavage fluid. (c) Inflammatory cell infiltration in lung tissues was observed by H&E. Scale bar = 100 μm. . . Data are representative of at least three separate experiments.

(a)

(b)

(c)