Dexmedetomidine Attenuates LPS-Stimulated Alveolar Type II Cells’ Injury through Upregulation of miR-140-3p and Partial Suppression of PD-L1 Involving Inactivating JNK-Bnip3 Pathway

Figure 2

Effects of miR-140-3p overexpression on PD-1/PD-L1 expression and cell viability of RLE-6TN cells. (a) The relative expression of PD-1/PD-L1 in RLE-6TN cells transfected with miR-140-3p mimic. (b) The cell apoptosis of RLE-6TN cells transfected with miR-140-3p mimic detected by TUNEL. (c) Predicted complementary binding site of miR-140-3p in PD-L1 3′-UTR. (d) The relative luciferase activities were inhibited and transfected with the reporter vector WT PD-L1, not with the reporter vector Mut PD-L1. Data are presented as means ± SD (n = 3; compared to control , compared to LPS-induced ^#, compared to control ).

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